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Western blot analysis of extracts of various cell lines, using HSPB8 antibody (18-681) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPB8 antibody (18-681). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HSPB8 antibody (18-681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.

HSPB8 Antibody

CATALOG NUMBER: 18-681

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
M21
Positive Control 3:
HeLa
Positive Control 4:
Mouse uterus
Positive Control 5:
Mouse lung
Positive Control 6:
Rat heart
Predicted Molecular Weight:
Observed: 22kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSPB8
Additional Names:
HSPB8, heat shock 22 kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27 kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal muscular atrophy, adult, autosomal dominant, CRYAC, PP1629
Ncbi Gene Id Number:
26353
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-681

List Size:
100 uL

List Price:

$436.00

Datasheet
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