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Western blot analysis in Jurkat cell line lysates (35ug/lane).
HSPBP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

HSPBP1 Antibody

CATALOG NUMBER: 57-976

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse, Rat
Immunogen:
This HSPBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-97 amino acids from the N-terminal region of human HSPBP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSPBP1
Additional Names:
Hsp70-binding protein 1, HspBP1, Heat shock protein-binding protein 1, Hsp70-binding protein 2, HspBP2, Hsp70-interacting protein 1, Hsp70-interacting protein 2, HSPBP1, HSPBP
Protein Accession Number:
Q9NZL4
Protein Gi Number:
74734730
Ncbi Gene Id Number:
23640
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HSPBP1 inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
Background Reference 1:
Graner, M.W., et al. Cancer Sci. 100(10):1870-1879(2009)
Background Reference 2:
Evdonin, A., et al. Biol. Cell 101(6):351-360(2009)
Background Reference 3:
Souza, A.P., et al. Cell Stress Chaperones 14(3):301-310(2009)
Background Reference 4:
Howarth, J.L., et al. J. Neurochem. 108(4):945-951(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-976

List Size:
400 ul

List Price:

$495.00

Datasheet
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