Western blot analysis of extracts of various cell lines, using HSPH1 antibody (22-359) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat spleen using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat fallopian tube using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using HSPH1 antibody (22-359) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

HSPH1 Antibody

CATALOG NUMBER: 22-359

Clonality:

Polyclonal

Tested Applications:

IHC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 659-858 of human HSPH1 (NP_006635.2).

Conjugate:

Unconjugated

Tested Applications:

IHC, WB

Application Note:

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Positive Control 1:

MCF7

Positive Control 2:

BT-474

Positive Control 3:

HepG2

Positive Control 4:

293T

Positive Control 5:

U-251MG

Positive Control 6:

Mouse brain

Predicted Molecular Weight:

Observed: 120kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

HSPH1

Additional Names:

Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, HSPH1, HSP105, HSP110, KIAA0201

Ncbi Gene Id Number:

10808

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-359

List Size:

100 uL

List Price:

$436.00

Datasheet

Shipping Info

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HSPH1 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Positive Control 4:

Positive Control 5:

Positive Control 6:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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