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Antibody used in WB on Human heart at 0.2-1 ug/ml.
Antibody used in IF on Mouse Spinal Cord at 1.3 ug/ml.
Antibody used in WB on Human heart at 0.2-1 ug/ml.

HTR3E Antibody

CATALOG NUMBER: 25-268

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HTR3E.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HTR3E antibody can be used for detection of HTR3E by ELISA at 1:312500. HTR3E antibody can be used for detection of HTR3E by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Predicted Molecular Weight:
53 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HTR3E antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HTR3E
Additional Names:
HTR3E, 5-HT3c1, MGC120035, MGC120036, MGC120037, 5-HT3E, 5-HT3-E
Protein Accession Number:
NP_872395
Protein Gi Number:
47519913
Ncbi Gene Id Number:
285242
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HTR3E belongs to the ligand-gated ion channel receptor superfamily. HTR3E is a subunit E of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. Genes encoding subunits C, D and E form a cluster on chromosome 3. The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes a subunit E of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. Genes encoding subunits C, D and E form a cluster on chromosome 3. An alternative splice variant has been described but its full length sequence has not been determined.
Background Reference 1:
Peters, J.A., Biochem. Soc. Trans. 32 (PT3), 547-552 (2004).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-268

List Size:
100 ul

List Price:

$519.00

Datasheet
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