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Recombinant Human Alkaline phosphatase (Intestinal type)/ALPI Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >10412.60 pmol/min/μg.
Recombinant Human Alkaline phosphatase (Intestinal type)/ALPI Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human Alkaline phosphatase (Intestinal type)/ALPI Recombinant Protein (85-114)

CATALOG NUMBER: 85-114

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Val20-Thr502
Fusion Tag:
C-6His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
53.15 kDa
Biological Activity:
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >10412.60 pmol/min/μg.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
ALPI, Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, EC:3.1.3.1
Protein Accession Number:
NP_001622.2
Ncbi Gene Id Number:
248
Background:
Recombinant Human Alkaline phosphatase (Intestinal type)/ALPI Protein encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-114

List Size:
0.02 mg, 0.05 mg

List Price:

Price range: $210.00 through $315.00

Datasheet
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