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Recombinant Human Alkaline phosphatase (Placental type) /ALPP Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human Alkaline phosphatase (Placental type) /ALPP Protein is greater than 95% as determined by SEC-HPLC.
Immobilized Human Alkaline Phosphatase (Placental type) , His Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC<sub>50</sub> of 11.1 ng/mL determined by ELISA.
Recombinant Human Alkaline phosphatase (Placental type) /ALPP Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human Alkaline phosphatase (Placental type) /ALPP Recombinant Protein (86-048)

CATALOG NUMBER: 86-048

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Fusion Tag:
C-6His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
53.8 kDa
Biological Activity:
Immobilized Human Alkaline Phosphatase (Placental type) , His Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 11.1 ng/mL determined by ELISA.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.2mM CaCl2,pH 7.5.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
ALPP, PLAP,Alkaline phosphatase, placental type, EC:3.1.3.1, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
Ncbi Gene Id Number:
250
Background:
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

86-048

List Size:
0.02 mg, 0.05 mg

List Price:

Price range: $182.00 through $280.00

Datasheet
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