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Recombinant Human Alpha-fetoprotein/AFP Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human AFP at 2μg/mL (100 μL/well) can bind AFP Mouse mAb with a linear range of 0.061-5.431 ng/mL.
Recombinant Human Alpha-fetoprotein/AFP Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human Alpha-fetoprotein/AFP Recombinant Protein (85-021)

CATALOG NUMBER: 85-021

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Arg19-Val609
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
67.32 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human AFP at 2 μg/mL (100 μL/well) can bind AFP Mouse mAb with a linear range of 0.061-5.431 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
AFPD,FETA,HPAFP,AFP
Protein Accession Number:
NP_001125.1
Ncbi Gene Id Number:
174
Background:
AFP is Alpha-fetoprotein classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-021

List Size:
0.05 mg

List Price:

$350.00

Datasheet
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