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Recombinant Human B7-H1/PD-L1/CD274 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human PD-L1/B7-H1 Protein (Cat.84-854) was greater than 95% as determined by SEC-HPLC.
Immobilized Human PD-L1 (Catalog: 84-854) at 200ng/mL (100 μL/well) can bind PD-L1/CD274 Rabbit mAb (Catalog: 81-687) with a linear range of 0.06-1.25 ng/mL.
Immobilized Recombinant Human PD-L1 His Tag (Catalog: 84-854) at 5ug/mL (100uL/well) can bind Recombinant Human PD-1 with a linear range of 0.16-1.09 μg/mL.
Recombinant Human B7-H1/PD-L1/CD274 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human B7-H1/PD-L1/CD274 Recombinant Protein (84-854)

CATALOG NUMBER: 84-854

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Phe19-Thr239
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
26.14 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PD-L1 at 10 μg/mL (100 μL/well) can bind Recombinant Human PD-1 with a linear range of 0.25-1.02 μg/mL.
2.Measured by its binding ability in a functional ELISA.Immobilized PE anti-human CD274 (B7-H1, PD-L1) Antibody at 1μg/mL (25 μL/well) can bind Human CD274 with a linear range of 0.46-18ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
B7-H, B7H1, PDL1, PD-L1, hPD-L1, PDCD1L1, PDCD1LG1,CD274,PDL1,B7H1,PD-L1,PDCD1L1,PDCD1LG1, B7-H, CD274 molecule
Protein Accession Number:
NP_054862.1
Ncbi Gene Id Number:
29126
Background:
This protein is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-854

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $112.00 through $280.00

Datasheet
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