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Recombinant Human Dkk-1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human DKK-1 at 5 μg/mL (100 μL/well) can bind Human LRP-5 with a linear range of 0.027-0.559 μg/mL.
Recombinant Human Dkk-1 inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The EC<sub>50</sub> for this effect is approximately 95 ng/ml in the presence of 2 μg/mL of Recombinant Human Wnt3a.
Recombinant Human Dkk-1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human Dkk-1 Recombinant Protein (85-404)

CATALOG NUMBER: 85-404

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Thr32-His266
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
26.61 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA.Immobilized Human DKK-1 at 5 μg/mL (100 μL/well) can bind Human LRP-5 with a linear range of 0.027-0.559 μg/mL.
2.Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The EC50 for this effect is approximately 95 ng/ml in the presence of 2 μg/mL of Recombinant Human Wnt3a.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
DKK1,DKK-1,SK
Protein Accession Number:
NP_036374.1
Ncbi Gene Id Number:
22943
Background:
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-404

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $112.00 through $252.00

Datasheet
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