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Recombinant Human DNMT1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 μM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 μM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent 85-858, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data)
Recombinant Human DNMT1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human DNMT1 Recombinant Protein (85-858)

CATALOG NUMBER: 85-858

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Pro2-Asp1632
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
185.53 kDa
Biological Activity:
The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 μM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 μM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent RP01868LQ, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data)
Purity:
≥ 85% as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Supplied as 0.22 μm filtered solution in 25mM Hepes-NaOH,500mM NaCl,0.04%, tritonX 100, 0.5mM TCEP, 10%glycerol,pH7.5.
Storage Conditions:
Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Additional Names:
DNMT1, AIM, CXXC9, DNMT,DNA (cytosine-5)-methyltransferase 1, Dnmt1, 2.1.1.37, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT
Protein Accession Number:
NP_001124295.1
Ncbi Gene Id Number:
1786
Background:
DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-858

List Size:
0.01 mg, 0.1 mg

List Price:

Price range: $210.00 through $1,120.00

Datasheet
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