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Recombinant Human E-Cadherin/CDH1/CD324 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured by its binding ability in a functional ELISA. Immobilized Human CDH1 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 (Catalog: 85-336) with a linear range of 62.50-218.35 ng/mL.
Recombinant Human E-Cadherin/CDH1/CD324 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human E-Cadherin/CDH1/CD324 Recombinant Protein (85-478)

CATALOG NUMBER: 85-478

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
Source Species:
HEK293 cells
Species:
Human
Source Species:
HEK293 cells
Recombinant Protein Sequence:
Asp155-Ala709
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
61.36 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Human CDH1 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 with a linear range of 62.50-218.35 ng/mL. 2.Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), > 28.35% will adhere specifically after 90 minutes at 37 °C.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Physical State:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Arc-1,CD324,CDHE,ECAD,LCAM,UVO,CDH1,E-Cadherin, Arc-1, CD324, CDHE, ECAD, LCAM, UVO, cadherin-1,E Cadherin
Protein Accession Number:
NP_004351.1
Ncbi Gene Id Number:
999
Background:
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-478

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $112.00 through $252.00

Datasheet
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