Western blot analysis of various lysates using Human EG-VEGF/PK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

Immunofluorescence analysis of paraffin-embedded Rat placenta tissue using Human EG-VEGF/PK1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded Human placenta tissue using Human EG-VEGF/PK1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using Human EG-VEGF/PK1 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

Human EG-VEGF/PK1 Rabbit Recombinant Antibody (81-444)

CATALOG NUMBER: 81-444

Clonality:

Monoclonal

Tested Applications:

ELISA, IF-P, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Recombinant protein (or fragment).This information is considered to be commercially sensitive.

Conjugate:

Unconjugated

Tested Applications:

ELISA, IF-P, WB

Application Note:

WB,1:500 - 1:1000, IF-P,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Positive Control 1:

293T,HepG2,Mouse liver,Mouse kidney,Rat ovary,Rat kidney

Predicted Molecular Weight:

12kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

PROK1

Additional Names:

PK1; PRK1; EGVEGF; PROK1

Protein Accession Number:

P58294

Ncbi Gene Id Number:

84432

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

81-444

List Size:

100 uL

List Price:

$477.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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Human EG-VEGF/PK1 Rabbit Recombinant Antibody (81-444)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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