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Recombinant Human EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human EGF enhances EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.
Immobilized Human EGFR at 5 μg/mL (100 μL/well) can bind Human EGF with a linear range of 7-25ng/mL.
Recombinant Human EGF promotes the proliferation of BALB/c 3T3 mouse embryonic fibroblasts cells. The ED<sub>50</sub> for this effect is typically 0.065-0.26 ng/mL, corresponding to a specific activity of 3.84×10<sup>6</sup>-1.54×10<sup>7</sup>units/mg.
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED<sub>50</sub> for this effect is 1.23-4.93 ng/mL, corresponding to a specific activity of 2.03×10<sup>5</sup>~8.13×10<sup>5</sup> units/mg.
Recombinant Human EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human EGF Recombinant Protein (85-187)

CATALOG NUMBER: 85-187

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Asn 971-Arg 1023
Fusion Tag:
C-hFc&His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
33.01 kDa
Biological Activity:
1.Recombinant Human EGF enhances EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.2.Immobilized Human EGFR at 5 μg/mL (100 μL/well) can bind Human EGF with a linear range of 7-25ng/mL.3.Recombinant Human EGF promotes the proliferation of BALB/c 3T3 mouse embryonic fibroblasts cells. The ED50 for this effect is typically 0.065-0.26 ng/mL, corresponding to a specific activity of 3.84×106-1.54×107units/mg.4.Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 1.23-4.93 ng/mL, corresponding to a specific activity of 2.03×105~8.13×105 units/mg.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
EGF,HOMG4,URG
Protein Accession Number:
NP_001954.2
Ncbi Gene Id Number:
1950
Background:
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin binding EGF like growth factor (HB_x005f

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-187

List Size:
0.05 mg

List Price:

$112.00

Datasheet
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