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Recombinant Human EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED<sub>50</sub> for this effect is 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×10<sup>6</sup>~2.50×10<sup>7</sup> units/mg.
Human liver organoids were cultured with EGF(Cat. 86-064), HGF(Cat. 85-644), FGF2(Cat. 85-199), FGF10(Cat. 85-242), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a.
Human kidney organoids were cultured with EGF(Cat. 86-064), FGF2(Cat. 85-199), FGF7(Cat. 85-732), FGF9(Cat. 85-725), FGF10(Cat. 85-242), IGF-(Cat. 85-173), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a. And further, DKK-1(85-404) was used to induce the establishment of cell polarity.
Human colorectal cancer organoid were cultured with EGF(Cat. 86-064), NOG(Cat. 85-332), FGF10(Cat. 85-242), RSPO1(Cat. 84-857), HGF(Cat. 85-644).
Human gastric cancer organoid were cultured with EGF(Cat. 86-064), NOG(Cat. 85-332), FGF10(Cat. 85-242), RSPO1(Cat. 84-857), Wnt-3a Surrogate.
Mouse lung organoids were cultured in Matrigel with medium supplemented with EGF (Cat. 86-064), Noggin (Cat. 85-332), RSPO1 (Cat. 84-857), Wnt-3a, and FGF-10 (Cat. 85-242).
Recombinant Human EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human EGF Recombinant Protein (86-064)

CATALOG NUMBER: 86-064

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Asn971-Arg1023
Fusion Tag:
No-Tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
6.35 kDa
Biological Activity:
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2ng/ml.
Purity:
≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by HPLC.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
EGF,Pro-epidermal growth factor, EGF, Cleaved into: Epidermal growth factor, Urogastrone
Protein Accession Number:
NP_001954.2
Ncbi Gene Id Number:
1950
Background:
As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

86-064

List Size:
0.05 mg

List Price:

$112.00

Datasheet
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