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Recombinant Human FGF-1/aFGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of human FGF acidic/FGF1 Protein (Cat.85-337) was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant Human FGF1 at 5μg/mL (100 μL/well) can bind Recombinant Human FGFR3 with a linear range of 0.5-1.5 μg/mL.
Recombinant Human FGF1 promotes the proliferation of BALB/c 3T3 mouse embryonic fibroblasts cells. The ED<sub>50</sub> for this effect is typically 22-140 pg/mL.
Recombinant Human FGF-1/aFGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human FGF-1/aFGF Recombinant Protein (85-337)

CATALOG NUMBER: 85-337

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
Source Species:
E. coli
Species:
Human
Source Species:
E. coli
Recombinant Protein Sequence:
Phe16-Asp155
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
16.67 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FGF1 at 5 μg/mL (100 μL/well) can bind Recombinant Human FGFR3 with a linear range of 0.5-1.5 μg/mL.
2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 22-140 pg/mL.
Purity:
≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Physical State:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 50mM MOPS,100mM Na2SO4,10mM EDTA, pH7.9Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1,FGF1,ECGF,ECGF-beta,ECGFA,ECGFB,FGF-1,FGF-alpha,FGFA,GLIO703,HBGF-1,HBGF1
Protein Accession Number:
NP_000791.1
Ncbi Gene Id Number:
2246

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-337

List Size:
0.05 mg

List Price:

$168.00

Datasheet
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