Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Recombinant Human FGF-2/bFGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant human FGF2 at 1 μg/mL (100 μL/well) can bind recombinant human FGFR4 with a linear range of 30-125 ng/mL.
Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20ng/mL.
Recombinant Human FGF-2 promotes the proliferation of Balb3T3 mouse embryonic fibroblasts cells. The ED<sub>50</sub> for this effect is 0.05-0.21 ng/mL, corresponding to a specific activity of 4.76×10<sup>6</sup>~2.00×10<sup>7</sup> units/mg.
Human liver organoids were cultured with EGF(Cat. 86-064), HGF(Cat. 85-644), FGF2(Cat. 85-199), FGF10(Cat. 85-242), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a.
Human kidney organoids were cultured with EGF(Cat. 86-064), FGF2(Cat. 85-199), FGF7(Cat. 85-732), FGF9(Cat. 85-725), FGF10(Cat. 85-242), IGF-(Cat. 85-173), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a. And further, DKK-1(85-404) was used to induce the establishment of cell polarity.
Recombinant Human FGF-2/bFGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human FGF-2/bFGF Recombinant Protein (85-199)

CATALOG NUMBER: 85-199

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Pro143-Ser288
Fusion Tag:
No tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
16.41 kDa
Biological Activity:
1.Immobilized recombinant human FGF2 at 1 μg/mL (100 μL/well) can bind recombinant human FGFR4 with a linear range of 30-125 ng/mL.
2.Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20ng/mL.
3.Recombinant Human FGF-2 promotes the proliferation of Balb3T3 mouse embryonic fibroblasts cells. The ED50 for this effect is 0.05-0.21 ng/mL, corresponding to a specific activity of 4.76×106~2.00×107 units/mg.
4.Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).
5.Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150 mM NaCl,pH7.5.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
BFGF, FGF-2, FGFB, HBGF-2,FGF2,FGF-2,FGFB,HBGF-2,Basic FGF, BFGF, fibroblast growth factor 2
Protein Accession Number:
NP_001997.5
Ncbi Gene Id Number:
2247
Background:
This protein is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-199

List Size:
0.02 mg, 0.05 mg

List Price:

Price range: $84.00 through $140.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369