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Recombinant Human FGF-7/HBGF-7/KGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED<sub>50</sub> for this effect is 2.19-8.76 ng/mL, corresponding to a specific activity of 1.14×10<sup>5</sup>~4.57×10<sup>5</sup> units/mg.
Recombinant Human FGF-7/HBGF-7/KGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human FGF-7/HBGF-7/KGF Recombinant Protein (85-732)

CATALOG NUMBER: 85-732

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Cys32-Thr194
Fusion Tag:
C-6His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.72 kDa
Biological Activity:
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 2.19-8.76 ng/mL, corresponding to a specific activity of 1.14×105~4.57×105 units/mg.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
KGF; HBGF-7;FGF7
Protein Accession Number:
NP_002000.1
Ncbi Gene Id Number:
2252
Background:
Fibroblast growth factor 7 (FGF7) is a member of the fibroblast growth factor (FGF) family of proteins. FGF7 plays an important role in regulating the proliferation, migration, and differentiation of cells. FGF7 is of stromal origin and produces a paracrine effect on epithelial cells. FGF7 is a mesenchyme-specific heparin-binding growth factor that binds FGF receptor 2 (FGFR2) to regulate numerous cellular and physiological processes. FGF7/FGFR2 promotes invasion and migration in human gastric cancer. FGF7 is specifically utilized as a paracrine factor during the process of differentiation of the epidermal layers in the regenerating scales and in particular for beta-cells differentiation. FGF7 over expression is associated with advanced clinical features in patients with upper tract and bladder urothelial carcinoma, justifying its potential prognostic value for urothelial carcinoma.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-732

List Size:
0.01 mg

List Price:

$140.00

Datasheet
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