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Recombinant Human Hepatocyte growth factor/HGF Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Recombinant Human Hepatocyte growth factor/HGF Protein is greater than 95% as determined by SEC-HPLC.
Recombinant Human HGF induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED<sub>50</sub> for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×10<sup>5</sup>-4.48×10<sup>5</sup> units/mg.
Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED<sub>50</sub> for this effect is 2.66-10.64 ng/mL,corresponding to a specific activity of 9.40×10<sup>4</sup>~3.76×10<sup>5</sup> units/mg.
Human liver organoids were cultured with EGF(Cat. 86-064), HGF(Cat. 85-644), FGF2(Cat. 85-199), FGF10(Cat. 85-242), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a.
Human colorectal cancer organoid were cultured with EGF(Cat. 86-064), NOG(Cat. 85-332), FGF10(Cat. 85-242), RSPO1(Cat. 84-857), HGF(Cat. 85-644), A83-01, SB202190, Y-27632 dihydrochloride, Acetylcysteine/NAC, Nicotinamide, Gastrin , AB-27 Supplement.
Recombinant Human Hepatocyte growth factor/HGF Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Human Hepatocyte growth factor/HGF Recombinant Protein (85-644)

CATALOG NUMBER: 85-644

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
Source Species:
HEK293 cells
Species:
Human
Source Species:
HEK293 cells
Recombinant Protein Sequence:
Gln32-Ser728
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
80.51 kDa
Biological Activity:
1.Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×105-4.48×105 units/mg.2.Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is 2.66-10.64 ng/mL,corresponding to a specific activity of 9.40×104~3.76×105 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Physical State:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
HGF,DFNB39,F-TCF,HGFB,HPTA,SF
Protein Accession Number:
NP_000592.3
Ncbi Gene Id Number:
3082
Background:
Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenicfactor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PANdomain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activatinga tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted bymesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability tostimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis,and tissue regeneration.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-644

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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