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Recombinant Human HMGB1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human HMGB1 (Cat: 84-807) at 2μg/mL (100 μL/well) can bind Human AGER/RAGE (Cat: 84-938) with a linear range of 0.1-42.8 ng/mL.
Recombinant Human HMGB1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human HMGB1 Recombinant Protein (84-807)

CATALOG NUMBER: 84-807

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Met1-Glu215
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
25.74 kDa
Biological Activity:
Immobilized Human HMGB1 at 2μg/mL (100 μL/well) can bind Human AGER/RAGE with a linear range of 0.1-42.8 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
HMG-1,HMG1,HMG3,SBP-1,HMGB1
Protein Accession Number:
NP_002119.1
Ncbi Gene Id Number:
3146
Background:
High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity. HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide. HMGB1 binds DNA in a non-sequence specific manner and may act as a structural cofactor during gene transcription.Acetylation of HMGB1 results in its cytoplasmic localization and eventual secretion. HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-807

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
Shipping Info

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