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Recombinant Human IFN-alpha B2/IFNA8 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IFN-alpha B2/IFNA8 was measured in a cell cytotoxicity assay using TF-1 cells. The ED<sub>50</sub> for this effect is 0.10-0.42 ng/mL, corresponding to a specific activity of 2.38×10<sup>6</sup>~1.0×10<sup>7</sup> units/mg.
Recombinant Human IFN-alpha B2/IFNA8 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IFN-alpha B2/IFNA8 Recombinant Protein (86-032)

CATALOG NUMBER: 86-032

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ser24-Glu189
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
20.58 kDa
Biological Activity:
Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.10-0.42 ng/mL, corresponding to a specific activity of 2.38×106~1.0×107 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IFNA8; IFN-alpha 8; IFNalpha B2; IFN-alpha B2; IFN-alpha-8; IFN-alphaB; interferon alpha type 201; interferon alpha-8; Interferon alpha-B; interferon alpha-B'; Interferon alpha-B2; interferon, alpha 8; LeIF B
Protein Accession Number:
NP_002161.2
Ncbi Gene Id Number:
3445
Background:
Interferon alpha-B, also known as IFNA8, belongs to the alpha/beta interferon family. Interferons are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites, or tumor cells. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They also allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. Interferons also activate immune cells, such as natural killer cells and macrophages. They increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes. They also increase the ability of uninfected host cells to resist new infections by virus. Certain symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Produced by macrophages, IFN-alpha has antiviral activities.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

86-032

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $126.00 through $280.00

Datasheet
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