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Recombinant Human IGF-I Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IGF1 enhances p70S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells.0.01-1ng/mL of Recombinant Human IGF1 can effectively enhance p70S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.
Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1μg/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250ng/mL.
Recombinant Human IGF1 promotes the proliferation of MCF-7 cells. The ED<sub>50</sub> for this effect is typically 7.5-30 ng/mL.
Recombinant Human IGF-I Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IGF-I Recombinant Protein (85-173)

CATALOG NUMBER: 85-173

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gly 49 - Ala 118
Fusion Tag:
C-hFc&His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
34.44 kDa
Biological Activity:
1.Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells. 0.01-1 ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.
2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 μg/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250 ng/mL. 3.Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 7.5-30 ng/mL, corresponding to a specific activity of 3.33×104-1.33×105units/mg.
Purity:
≥ 85 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IGF1,IGF-I,IGFI,MGF, IGFI, MGF
Protein Accession Number:
NP_001104755.1
Ncbi Gene Id Number:
3479

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-173

List Size:
0.05 mg

List Price:

$140.00

Datasheet
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