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Recombinant Human IGFBP-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human IGFBP4 at 1 μg/mL (100 μL/well) can bind Human IGF1 with a linear range of 1.95-111.32 ng/mL.
Immobilized Human IGFBP4 Protein at 1 μg/mL (100 μL/well) can bind IGF1 with a linear range of 1.95-122.3ng/mL.
Recombinant Human IGFBP-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IGFBP-4 Recombinant Protein (84-968)

CATALOG NUMBER: 84-968

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Met1-Glu258
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
28.77 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 at 1 μg/mL (100 μL/well) can bind Human IGF1 with a linear range of 1.95-111.32 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 Protein at 1 μg/mL (100 μL/well) can bind IGF1 with a linear range of 1.95-122.3ng/mL.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IGFBP4,BP-4,HT29-IGFBP,IBP4,IGFBP-4
Protein Accession Number:
NP_001543.2
Ncbi Gene Id Number:
3487
Background:
IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins . IGFBP-4 binds both insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. It circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. There is mounting evidence that IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-968

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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