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Recombinant Human IL-1 alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 9.39-37.58 pg/mL, corresponding to a specific activity of 2.66×10<sup>7</sup>~1.06×10<sup>8</sup> units/mg.
Recombinant Human IL-1 alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-1 Alpha Recombinant Protein (86-065)

CATALOG NUMBER: 86-065

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Ser113-Ala271
Fusion Tag:
NO-Tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
18.05 kDa
Biological Activity:
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 9.39-37.58 pg/mL, corresponding to a specific activity of 2.66×107~1.06×108 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
Please contact us for more information.
physical-state:
Lyophilized
Buffer:
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL1A, IL1F1, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1
Protein Accession Number:
NP_000566.3
Ncbi Gene Id Number:
3552
Background:
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

86-065

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
Shipping Info

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