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Recombinant Human IL-1 beta Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IL-1 beta Protein is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-1 beta (Catalog: 84-800) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: 85-193) with a linear range of 0.002-1 μg/mL.
Recombinant Human IL-1 beta promotes the proliferation of D10.G4.1 mouse helper T cells. The ED<sub>50</sub> for this effect is 6.04-24.16 pg/mL, corresponding to a specific activity of 4.14×10<sup>7</sup>~1.66×10<sup>8</sup> units/mg.
Recombinant Human IL-1 beta Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-1 beta Recombinant Protein (84-800)

CATALOG NUMBER: 84-800

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Ala117-Ser269
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
18.22 kDa
Biological Activity:
1.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 6.04-24.16 pg/mL, corresponding to a specific activity of 4.14×107~1.66×108 units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 with a linear range of 0.002-1 μg/mL.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL-1,IL1-BETA,IL1F2,IL1 beta,IL1B
Protein Accession Number:
NP_000567.1
Ncbi Gene Id Number:
3553
Background:
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-800

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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