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Recombinant Human IL-2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using CTLL-2 mouse T cells. The ED<sub>50</sub> for this effect is 0.255-1.02 ng/mL,corresponding to a specific activity of 9.80×10<sup>5</sup>~3.92×10<sup>6</sup>units/mg.
Recombinant Human IL2 stimulates cell proliferation of the HT-2 Mouse T cells. The ED<sub>50</sub> for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 10<sup>6</sup>~4.0× 10<sup>6</sup> units/mg.
Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.
Recombinant Human IL-2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-2 Recombinant Protein (85-196)

CATALOG NUMBER: 85-196

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala21-Thr153
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
16.26 kDa
Biological Activity:
1.Measured in a cell proliferation assay using CTLL-2 mouse T cells. The ED50 for this effect is 0.255-1.02 ng/mL,corresponding to a specific activity of 9.80×105~3.92×106units/mg.
2.Measured in a cell proliferation assay using HT-2 Mouse T cells. The ED50 for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 106~4.0× 106 units/mg.
3.Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA Protein at 2 μg/mL (100 μL/well) can bind Human IL2 with a linear range of 2.75-64.64 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL2,IL-2,TCGF,lymphokine
Protein Accession Number:
NP_000577.2
Ncbi Gene Id Number:
3558
Background:
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-196

List Size:
0.02 mg, 0.05 mg

List Price:

Price range: $126.00 through $168.00

Datasheet
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