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Recombinant Human IL-23/IL-12B&IL-23A Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Immobilized Human IL-12B&IL-23A at 5μg/mL (100 μL/well) can bind Human IL23R with a linear range of 4-135 ng/mL.
Recombinant Human IL-23/IL-12B&IL-23A induce IL-17 secretion by mouse splenocytes. The ED<sub>50</sub> for this effect is 21.23-84.94 ng/mL, corresponding to a specific activity of 1.17×10<sup>4</sup>~4.71×10<sup>4</sup> units/mg.
Recombinant Human IL-23/IL-12B&IL-23A Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Human IL-23/IL-12B&IL-23A Recombinant Protein (85-255)

CATALOG NUMBER: 85-255

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ile23-Ser328&Arg20-Pro189
Fusion Tag:
No tag(IL12B)&N-his(IL23A)
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.50 kDa(IL-12A), 34.71 kDa(IL-12B)
Biological Activity:
1.Measured by its binding ability in a functional ELISA.Immobilized Human IL-12B&IL-23A at 5μg/mL (100 μL/well) can bind Human IL23R with a linear range of 4-135 ng/ml.
2.Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 21.23-84.94 ng/mL, corresponding to a specific activity of 1.17×104~4.71×104 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL-23 p19/IL-12 p40,IL23,SGRF ,IL-23 alpha & IL-12 beta
Protein Accession Number:
NP_002178.2/NP_057668.1
Ncbi Gene Id Number:
3593&51561

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-255

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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