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Recombinant Human IL-27/IL-27A&IL-27B Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human IL-27 RA, hFc Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC<sub>50</sub> of 55.9ng/mL determined by ELISA (QC Test).
Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
Recombinant Human IL-27/IL-27A&IL-27B Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-27/IL-27A&IL-27B Recombinant Protein (85-104)

CATALOG NUMBER: 85-104

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Fusion Tag:
N-His & NO-tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
50.6 kDa
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C. Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL27A&IL27B,Interleukin-27 subunit alpha&Interleukin-27 subunit beta,IL-27 subunit alpha&IL-27 subunit beta,IL-27A&IL-27B
Protein Accession Number:
NP_663634.2
Ncbi Gene Id Number:
246778 & 10148
Background:
IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells, and dendritic cells. IL-27 protein is also referred to as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 , and IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives the rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-104

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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