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Recombinant Human IL-33 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Recombinant human IL33 at 10 μg/mL (100 μL/well) can bind Recombinant human IL1RL1 with a linear range of 37.8-151.4 ng/mL.
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED<sub>50</sub> for this effect is 0.1857-0.7428 ng/mL, corresponding to a specific activity of 1.3×10<sup>6</sup>~5.4×10<sup>6</sup> units/mg.
Recombinant Human IL-33 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-33 Recombinant Protein (84-814)

CATALOG NUMBER: 84-814

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Ser112-Thr270
Fusion Tag:
No tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
17.99 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant human IL33 at 10 μg/mL (100 μL/well) can bind Recombinant human IL1RL1 with a linear range of 37.8-151.4 ng/mL.
2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.1857-0.7428 ng/mL, corresponding to a specific activity of 1.3×106~5.4×106 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL33,C9orf26,DVS27,IL1F11,NF-HEV,NFEHEV
Protein Accession Number:
NP_001300973.1
Ncbi Gene Id Number:
90865
Background:
Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-814

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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