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Recombinant Human IL-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IL-4 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED<sub>50</sub> for this effect is 0.06-0.24 ng/mL, corresponding to a specific activity of 4.17×10<sup>6</sup>-1.67×10<sup>7</sup> units/mg.
Recombinant Human IL-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-4 Recombinant Protein (85-721)

CATALOG NUMBER: 85-721

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
His25-Ser153
Fusion Tag:
No-tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Biological Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.06-0.24 ng/mL, corresponding to a specific activity of 4.17×106-1.67×107 units/mg.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
BSF1; IL-4; BCGF1; BSF-1; BCGF-1;IL4
Protein Accession Number:
NP_000580.1
Ncbi Gene Id Number:
3565
Background:
Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-721

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
Shipping Info

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