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Recombinant Human IL-7 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using murine 2E8 cells. The ED<sub>50</sub> for this effect is 0.67-2.68 ng/mL, corresponding to a specific activity of 3.7×10<sup>5</sup>~1.49×10<sup>6</sup> units/mg.
Recombinant Human IL-7 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-7 Recombinant Protein (85-723)

CATALOG NUMBER: 85-723

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Asp26-His177
Fusion Tag:
No-tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Biological Activity:
1.Measured in a cell proliferation assay using IL-2-activated mouse spleen cell. The ED50 for this effect is 13-53 pg/mL, corresponding to a specific activity of 1.89×107~7.69×107 units/mg.
2.Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 2.2-8.7ng /mL, corresponding to a specific activity of 1.15×105~4.54×106 units/mg.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL-7,Interleukin-7;IL7
Protein Accession Number:
NP_000871.1
Ncbi Gene Id Number:
3574
Background:
IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCR?) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-723

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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