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Recombinant Human Lipocalin-2/NGAL/LCN2 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant mouse Lipocalin-2. Recombinant human Lipocalin-2 can bind >2.84 μM of Fe(DHBA)3.
Recombinant Human Lipocalin-2/NGAL/LCN2 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Human Lipocalin-2/NGAL/LCN2 Recombinant Protein (85-820)

CATALOG NUMBER: 85-820

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln21-Gly198
Fusion Tag:
C-hFC
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
46.51 kDa
Biological Activity:
1, Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant mouse Lipocalin-2. Recombinant human Lipocalin-2 can bind >2.84 μM of Fe(DHBA)3.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
p25; 24p3; MSFI; NGAL;Lipocalin-2;LCN2
Protein Accession Number:
NP_005555.2
Ncbi Gene Id Number:
3934
Background:
Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-820

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $126.00 through $280.00

Datasheet
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