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Recombinant Human MMP-1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human MMP-1 induces cell migration of the A549 cells. 1 ng/mL of Recombinant Human MMP-1 can effectively induce A549 cells migration.
Recombinant Human MMP-1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human MMP-1 Recombinant Protein (84-992)

CATALOG NUMBER: 84-992

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Phe20-Asn469
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
51.84 kDa
Biological Activity:
1.Measured in a cell migration assay using A549 cells. 1 ng/mL of Recombinant Human MMP-1 can effectively induce A549 cells migration.
2.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >997 pmol/min/μg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 25mM MES, 10mM CaCl2, 150mM NaCl, 0.05% Brij, pH 5.5.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
MMP1,CLG,CLGN
Protein Accession Number:
NP_002412.1
Ncbi Gene Id Number:
4312
Background:
MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages.Mutations of MMP1 are associated with chronic obstructive pulmonary disease (COPD).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-992

List Size:
0.01 mg

List Price:

$210.00

Datasheet
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