Coomassie blue staining of human normal tissue lysates in 12% SDS-PAGE. Lanes: 1, protein markers; 2, brain; 3, colon; 4, heart; 5, kidney; 6, liver; 7, lung; 8, pancreas; and 9, spleen.

Human Normal Tissue Blot I

CATALOG NUMBER: 1521

Tested Applications:

Specifications

Species:

Human

Samples Loaded:

Lanes: 1, protein markers; 2, brain; 3, colon; 4, heart; 5, kidney; 6, liver; 7, lung; 8, pancreas; and 9, spleen.

Tested Applications:

Application Note:

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.

Advanced Validation

Properties

Gel Percentage:

Gradient 4-20%

Storage Conditions:

Store at 4°C. The blot can be reused after stripping.

Additional Info

User Note:

Note: Lysate lane order may vary; please consult accompanying shipment paperwork.

Background

Background:

The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

1521

List Size:

One blot

List Price:

$429.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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Human Normal Tissue Blot I

Tested Applications:

Species:

Samples Loaded:

Tested Applications:

Application Note:

Gel Percentage:

Storage Conditions:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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