Human Normal Tissue Blot V

CATALOG NUMBER: 1525

Tested Applications:
WB
Specifications
Species:
Human
Samples Loaded:
Lanes: 1, protein markers; 2, appendix; 3, kidney; 4, liver; 5, lymph node; 6, peripheral blood leukocyte; 7, spleen; 8, thymus; 9, tonsil; and 10, thyroid
Tested Applications:
WB
Application Note:
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Gel Percentage:
Gradient 4-20%
Storage Conditions:
Store at 4°C. The blot can be reused after stripping.
User Note:
Note: Lysate lane order may vary; please consult accompanying shipment paperwork.
Background:
The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, and thyroid.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

1525

List Size:
One blot

List Price:

$429.00

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