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Recombinant Human PDGF-BB Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized PDGF-BB (Catalog: 85-771) at 2 μg/mL (100 μL/well) can bind Human PDGFRB (Catalog: 84-910) with a linear range of 0.1-75.4 ng/mL.
Recombinant Human PDGF-BB Protein was measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED<sub>50</sub> for this effect is 2.09-8.36 ng/mL, corresponding to a specific activity of 1.20×10<sup>5</sup>~4.78×10<sup>5</sup> units/mg.
Recombinant Human PDGF-BB Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human PDGF-BB Recombinant Protein (85-771)

CATALOG NUMBER: 85-771

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
Pichia
Species:
Human
source-species:
Pichia
Recombinant Protein Sequence:
Ser82-Thr190
Fusion Tag:
No-tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
12.30 kDa
Biological Activity:
1.Immobilized PDGF-BB at 2 μg/mL (100 μL/well) can bind Human PDGFRB with a linear range of 0.1-75.4 ng/mL.2.Recombinant Human PDGF-BB Protein was measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 2.09-8.36 ng/mL, corresponding to a specific activity of 1.20×105~4.78×105 units/mg.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 20mM NaAc, pH 4.5.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2;PDGF subunit B;PDGF-BB;PDGFB
Protein Accession Number:
NP_002599.1
Ncbi Gene Id Number:
5155
Background:
Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-771

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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