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Recombinant Human R-spondin-1/RSPO1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Recombinant Human R-spondin-1/RSPO1 Protein is greater than 90% as determined by SEC-HPLC.
Recombinant Human R-spondin-1/RSPO1 Protein induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 6.61~26.44 ng/mL in the presence of 5 ng/mL Wnt Surrogate Protein, corresponding to a specific activity of 3.78×10<sup>4</sup>~1.51×10<sup>5</sup> units/mg.
Human stomach organoids were cultured with EGF(Cat. 86-064), FGF10(Cat. 85-242), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a.
Mouse large intestinal organoids were cultured with EGF(Cat. 86-064), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a
Mouse small intestinal organoids were cultured with EGF(Cat. 86-064), NOG(Cat. 85-332), RSPO1(Cat. 84-857).
Human liver organoids were cultured with EGF(Cat. 86-064), HGF(Cat. 85-644), FGF2(Cat. 85-199), FGF10(Cat. 85-242), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a.
Human kidney organoids were cultured with EGF(Cat. 86-064), FGF2(Cat. 85-199), FGF7(Cat. 85-732), FGF9(Cat. 85-725), FGF10(Cat. 85-242), IGF-(Cat. 85-173), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a. And further, DKK-1(85-404) was used to induce the establishment of cell polarity.
Mouse lung organoids were cultured in Matrigel with medium supplemented with EGF (Cat. 86-064), Noggin (Cat. 85-332), RSPO1 (Cat. 84-857), Wnt-3a, and FGF-10 (Cat. 85-242).
Recombinant Human R-spondin-1/RSPO1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human R-spondin-1/RSPO1 Recombinant Protein (84-857)

CATALOG NUMBER: 84-857

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Arg31-Ala263
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
26.44 kDa
Biological Activity:
1.The intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071) derived from ABclonal for144 hours, intestinal organoids were formed. (Customer Feedback Data)
2.Recombinant Human R-Spondin 1 protein stimulated Wnt signal pathway with Wnt-3a protein in HEK293T cells. After 6 hours, the stimulation when adding 300 ng/mL of R-Spondin-1 reached highest effect. Compared with only Wnt-3a stimulation, the Wnt signaling pathway was enhanced 3.1-fold after adding 300 ng/mL R-Spondin 1.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
RSPO1,CRISTIN3,RSPO
Protein Accession Number:
NP_001033722.1
Ncbi Gene Id Number:
284654
Background:
This protein is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-857

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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