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Recombinant Human TNFRSF10B/DR5/TRAIL-R2/CD262 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human DR5/TRAIL R2 Protein (Cat.85-445) was greater than 90% as determined by SEC-HPLC.
Immobilized Human TNFRSF10B at 1 μg/mL (100 μL/well) can bind Human TNFSF10 with a linear range of 0.1-11.7 ng/mL.
Recombinant Human TNFRSF10B inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED<sub>50</sub> for this effect is 30-120 pg/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Recombinant Human TNFRSF10B/DR5/TRAIL-R2/CD262 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNFRSF10B/DR5/TRAIL-R2/CD262 Recombinant Protein (85-445)

CATALOG NUMBER: 85-445

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ile56-Glu182
Fusion Tag:
C-hFc&His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
41.07 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA.Immobilized Human TNFRSF10B at 1 μg/mL (100 μL/well) can bind Human TNFSF10 with a linear range of 0.1-11.7 ng/mL.
2.Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L_x001e_929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 30-120 pg/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
CD262,DR5,KILLER,KILLER/DR5,TRAIL-R2,TRAILR2,TRICK2,TRICK2A,TRICK2B,TRICKB,ZTNFR9,TNFRSF10B
Protein Accession Number:
NP_003833.3
Ncbi Gene Id Number:
8795
Background:
This protein is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-445

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $98.00 through $210.00

Datasheet
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