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Recombinant Human TNFRSF10D/DcR2/TRAIL-R4/CD264 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Recombinant Human TRAIL at 2μg/mL (100 μL/well) can bind Recombinant Human DcR2 with a linear range of 15-60 ng/mL.
Recombinant Human TNFRSF10D inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED<sub>50</sub> for this effect is 1.8-7.2 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Recombinant Human TNFRSF10D/DcR2/TRAIL-R4/CD264 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNFRSF10D/DcR2/TRAIL-R4/CD264 Recombinant Protein (85-169)

CATALOG NUMBER: 85-169

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala 56 - His 211
Fusion Tag:
C-hFc&His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
43.59 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TRAIL at 2 μg/mL (100 μL/well) can bind Recombinant Human DcR2 with a linear range of 15-60 ng/mL.
2.Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 1.8-7.2 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
CD264, DCR2, TRAIL-R4, TRAILR4, TRUNDD,TNFRSF10D,DCR2,TRAIL-R4,TRAILR4,TRUNDD
Protein Accession Number:
NP_003831.2
Ncbi Gene Id Number:
8793

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-169

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $98.00 through $210.00

Datasheet
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