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Recombinant Human TNFRSF1A/TNF-R1/CD120a Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant Mouse TNFα at 2 μg/mL (100 μL/well) can bind Human TNFRSF1A with a linear range of 4-53 ng/mL.
Recombinant Human TNFRSF1A inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is typically 14-57 ng/mL in the presence of 0.25 ng/mL recombinant human TNF-α.
Recombinant Human TNFRSF1A/TNF-R1/CD120a Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNFRSF1A/TNF-R1/CD120a Recombinant Protein (85-469)

CATALOG NUMBER: 85-469

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Leu30-Thr211
Fusion Tag:
C-hFc
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
46.45 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA.Immobilized Mouse TNFα at 2 μg/mL (100 μL/well) can bind Human TNFRSF1A with a linear range of 4-53 ng/mL.
2.Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 14-57 ng/mL in the presence of 0.25 ng/mL recombinant human TNF-α.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
TNFRSF1A,CD120a,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR1,TNFR55,TNFR60,p55,p55-R,p60
Protein Accession Number:
NP_001056.1
Ncbi Gene Id Number:
7132
Background:
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-469

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $126.00 through $280.00

Datasheet
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