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Recombinant Human TNFRSF1B/TNF-R2/CD120b Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human TNFR2 inhibits TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is typically 1-3μg/mL in the presence of 0.25 ng/mL recombinant human TNF-α.
Recombinant Human TNFRSF1B/TNF-R2/CD120b Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNFRSF1B/TNF-R2/CD120b Recombinant Protein (85-030)

CATALOG NUMBER: 85-030

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Leu 23 - Asp 257
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
25.97 kDa
Biological Activity:
Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-3 μg/mL in the presence of 0.25 ng/mL recombinant human TNF-α.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR,TNF Receptor II,TNFRSF1B
Protein Accession Number:
NP_001057.1
Ncbi Gene Id Number:
7133

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-030

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $196.00 through $448.00

Datasheet
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