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Recombinant Human TNFRSF6/FAS/CD95 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant Human Fas Ligand at 2μg/mL (100 μL/well) can bind recombinant Human FAS, the EC<sub>50</sub> of Human FAS is 6.23 ng/mL.
Recombinant Human FAS inhibit Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells. The ED<sub>50</sub> for this effect is typically 16.5-66 ng/mL in the presence of 5 ng/mL Recombinant Human Fas Ligand.
Recombinant Human TNFRSF6/FAS/CD95 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNFRSF6/FAS/CD95 Recombinant Protein (84-923)

CATALOG NUMBER: 84-923

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln26-Asn173
Fusion Tag:
C-hFc&His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
43.43 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Human Fas Ligand at 2 μg/mL (100 μL/well) can bind recombinant Human FAS. The EC50 of Human FAS is 6.23 ng/mL.
2.Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells. The ED50 for this effect is typically 16.5-66 ng/mL in the presence of 5 ng/mL Recombinant Human Fas Ligand.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
ALPS1A,APO-1,APT1,CD95,FAS1,FASTM,TNFRSF6,FAS, ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, Fas cell surface death receptor
Protein Accession Number:
NP_000034.1
Ncbi Gene Id Number:
355
Background:
The protein is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-923

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $126.00 through $280.00

Datasheet
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