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Recombinant Human TNFSF13B/BAFF/CD257 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant human BAFF at 5 μg/mL (100 μL/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL.
Recombinant Human BAFF induces mouse B cell proliferation. The ED<sub>50</sub> for this effect is 0.1-1 ng/mL, corresponding to a specific activity of 1×10<sup>6</sup>-1×10<sup>7</sup>units/mg.
Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His (Catalog: 84-939) on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag (Catalog: 84-813) with an affinity constant of 3.05nM as determined in BLI assay (Gator).
Recombinant Human TNFSF13B/BAFF/CD257 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNFSF13B/BAFF/CD257 Recombinant Protein (84-813)

CATALOG NUMBER: 84-813

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Ala134-Leu285
Fusion Tag:
No tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
17.04 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 5 μg/mL (100 μL/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL.
2.Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1-1 ng/mL, corresponding to a specific activity of 1×106-1×107units/mg.
3.Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag with an affinity constant of 3.05nM as determined in BLI assay (Gator).
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
Please contact us for more information.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
TNFSF13B,BAFF,BLYS,CD257,DTL,TALL-1,TALL1,THANK,TNFSF20,TNLG7A,ZTNF4
Protein Accession Number:
NP_006564.1
Ncbi Gene Id Number:
10673
Background:
The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-813

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $126.00 through $280.00

Datasheet
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