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Recombinant Human VEGF-A/VEGF165 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
The purity of human VEGF165 Protein (Cat.85-246) was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant Human VEGF165 at 1 μg/mL (100 μL/well) can bind Recombinant Human VEGFR2 with a linear range of 8-20 ng/mL.
Immobilized Recombinant Human VEGF165 at 2 μg/mL (100 μL/well) can bind Human KDR with a linear range of 0.2-11.6 ng/mL.
Recombinant Human VEGF165 stimulates cell proliferation of the human umbilical vein endothelial cells (HUVEC). The ED<sub>50</sub> for this effect is typically 0.19-0.78 ng/mL, corresponding to a specific activity of 1.28 × 10<sup>6</sup>~5.26× 10<sup>6</sup> units/mg.
Recombinant Human VEGF-A/VEGF165 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Human VEGF-A/VEGF165 Recombinant Protein (85-246)

CATALOG NUMBER: 85-246

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala27-Arg191
Fusion Tag:
N-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
20.01 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGF165 at 1 μg/mL (100 μL/well) can bind Recombinant Human VEGFR2 with a linear range of 8-20 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 2 μg/mL (100 μL/well) can bind Human KDR with a linear range of 0.2-11.6 ng/mL.
3.Recombinant Human VEGF165 stimulates cell proliferation of the human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.19-0.78 ng/mL, corresponding to a specific activity of 1.28 × 106~5.26× 106 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
VEGFA, MVCD1, VEGF, VPF, vascular endothelial growth factor A,MVCD1,VEGF,VPF,L VEGFA,VEGF A
Protein Accession Number:
NP_001165097.1
Ncbi Gene Id Number:
7422

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-246

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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