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Recombinant Human Wnt-3a Surrogate Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human Wnt-3a Surrogate Protein induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED<sub>50</sub> for this effect is typically 3.95-15.79 ng/mL, corresponding to a specific activity of 6.33×10<sup>4</sup>~2.53×10<sup>5</sup> units/mg.
Recombinant Human Wnt-3a Surrogate Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human Wnt-3a Surrogate Recombinant Protein (85-656)

CATALOG NUMBER: 85-656

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Fusion Tag:
C-hFC
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
57.12 kDa
Biological Activity:
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 3.95-15.79 ng/mL, corresponding to a specific activity of 6.33×104~2.53×105 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-656

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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