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Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (16-418) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (16-418) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse testis using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using HuR / ELAVL1 antibody (16-418). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (16-418) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
KO

HuR / ELAVL1 Antibody, KO Validated

CATALOG NUMBER: 16-418

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
U-251MG
Positive Control 2:
HeLa
Positive Control 3:
Jurkat
Positive Control 4:
K-562
Positive Control 5:
PC-3
Predicted Molecular Weight:
Observed: 37kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ELAVL1
Additional Names:
ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG
Ncbi Gene Id Number:
1994
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-418

List Size:
100 uL

List Price:

$540.00

Datasheet
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