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Western blot analysis of HVCN1 in (A) human spleen and (B) mouse spleen tissue lysates with HVCN1 antibody at 1 μg/mL.
Immunohistochemistry of HVCN1 in rat spleen tissue with HVCN1 antibody at 5 μg/mL.
Immunofluorescence of HVCN1 in Rat Spleen cells with HVCN1 antibody at 20 μg/mL.
Western blot analysis of HVCN1 in (A) human spleen and (B) mouse spleen tissue lysates with HVCN1 antibody at 1 μg/mL.
Trial Size

HVCN1 Antibody

CATALOG NUMBER: 5875

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (71%)
Immunogen:
HVCN1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human HVCN1.
The immunogen is located within the last 50 amino acids of HVCN1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
HVCN1 antibody can be used for detection of HVCN1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 2:
Cat. No. 1306 - Human Spleen Tissue Lysate
Predicted Molecular Weight:
Predicted: 30 kDa
Observed: 30 kDa
Purification:
HVCN1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HVCN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HVCN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HVCN1
Additional Names:
HVCN1 Antibody: HV1, VSOP, UNQ578/PRO1140, Voltage-gated hydrogen channel 1, Hydrogen voltage-gated channel 1, HV1
Protein Accession Number:
NP_115745
Protein Gi Number:
91992155
Ncbi Gene Id Number:
84329
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HVCN1 Antibody: HVCN1 (hydrogen voltage-gated channel 1), a voltage gated proton channel protein that is highly expressed in immune tissues, mediates the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing. HVCN1 moderates the voltage-dependent proton permeability of excitable membranes by allowing the flow of protons in accordance to their electrochemical gradient. HVCN1 is sensitive to zinc ions, and can be inhibited by them. HVCN1 may represent a new therapeutic target for B cell malignancies on continued signaling via the B cell antigen receptor.
Background Reference 1:
Ramsey IS, Moran MM, Chong JA, et al. A voltage gated proton-selective channel lacking the pore domain. Nature2006; 440:1213-6.
Background Reference 2:
Sasaki M, Takagi M, and Okamura Y. A voltage sensor-domain protein is a voltage-gated proton channel. Science2006; 312: 589-92.
Background Reference 3:
Morgan D, Capasso M, and Musset B. Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis. Proc. Natl. Acad. Sci. USA2009; 106:18022-7.
Background Reference 4:
Capasso M, Bhamrah MK, Henley T, et al. HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species. Nat. Immunol.2010;11:265-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5875

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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