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45-753 (0.03ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-753 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-753 (0.03ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HVEM Antibody

CATALOG NUMBER: 45-753

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SVQRKRQEAEGE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Thymus and Tonsil lysates (calculated MW of 30.4kDa according to NP_003811.2). Recommended concentration: 0.03-0.1 ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TNFRSF14
Additional Names:
TNFRSF14, HVEM, TR2, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator), HGNC:11912, ATAR, HVEA, LIGHTR, CD40-like protein precursor, herpesvirus entry mediator, herpesvirus entry mediator A, tumor necrosis factor receptor superfamily, member 14, tumor necrosis factor receptor-like gene2
Protein Accession Number:
NP_003811.2
Protein Gi Number:
23200041
Ncbi Gene Id Number:
8764
Background Reference 1:
Cheung TC, Humphreys IR, Potter KG, Norris PS, Shumway HM, Tran BR, Patterson G, Jean-Jacques R, Yoon M, Spear PG, Murphy KM, Lurain NS, Benedict CA, Ware CF. From The Cover: Evolutionarily divergent herpesviruses modulate T cell activation by targeting the herpesvirus entry mediator cosignaling pathway. Proc Natl Acad Sci USA. 2005 Sep 13;102(37):13218-23. Epub 2005 Aug 30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-753

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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