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Western blot analysis of extracts of various cell lines, using Hydroxyl-Histone H2A-Y39 antibody (13-880).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse colon using Hydroxyl-Histone H2A-Y39 antibody (13-880) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Hydroxyl-Histone H2A-Y39 antibody (13-880) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Hydroxyl-Histone H2A-Y39 antibody (13-880) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Hydroxyl-Histone H2A-Y39 antibody (13-880) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using Hydroxyl-Histone H2A-Y39 antibody (13-880).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Hydroxyl-Histone H2A-Y39 Antibody [G7E5]

CATALOG NUMBER: 13-880

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A specific peptide of human Hydroxyl-Histone H2A-Y39
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:500 - 1:1000
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HIST1H2AE
Additional Names:
H2A.1, H2A.2, H2A/a, H2AFA, histone H2A type 1-B/E, H2A histone family, member A, histone 1, H2ae, histone H2A/a, histone H2AE, histone cluster 1, H2ae
Ncbi Gene Id Number:
3012
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-880

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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