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Western blot analysis of lysate from HepG2 cell line, using HYOU1 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of HYOU1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysate from HepG2 cell line, using HYOU1 Antibody at 1:1000 at each lane.

HYOU1 Antibody

CATALOG NUMBER: 62-818

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HYOU1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human HYOU1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
111 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HYOU1
Additional Names:
Hypoxia up-regulated protein 1, 150 kDa oxygen-regulated protein, ORP-150, 170 kDa glucose-regulated protein, GRP-170, HYOU1, GRP170, ORP150
Protein Accession Number:
Q9Y4L1
Protein Gi Number:
10720185
Ncbi Gene Id Number:
10525
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.
Background Reference 1:
Kitao,Y., Matsuyama,T. Antioxid. Redox Signal.(5), 589-595 (2007)
Background Reference 2:
Bando,Y., Ogawa,S. Am. J. Physiol., Cell Physiol. 278 (6), C1172-C1182 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-818

List Size:
400 ul

List Price:

$495.00

Datasheet
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