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Antibody staining I23O2 in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:4000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human kidney lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in K562 cell line lysates (35ug/lane).
I23O2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Antibody staining I23O2 in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

I23O2 Antibody

CATALOG NUMBER: 56-406

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This I23O2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-295 amino acids from the C-terminal region of human I23O2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IDO2
Additional Names:
Indoleamine 2,3-dioxygenase 2, IDO-2, 11311-, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1, IDO2, INDOL1
Protein Accession Number:
Q6ZQW0
Protein Gi Number:
215274147
Ncbi Gene Id Number:
169355
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Along with the enzymes encoded by the INDO (MIM 147435)and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 genemetabolizes tryptophan in the kynurenine pathway (Ball et al., 2007[PubMed 17499941]).
Background Reference 1:
Cetindere, T., et al. Cell. Signal. 22(2):197-211(2010)
Background Reference 2:
Huttunen, R., et al. Shock 33(2):149-154(2010)
Background Reference 3:
Witkiewicz, A.K., et al. J. Am. Coll. Surg. 208(5):781-787(2009)
Background Reference 4:
Metz, R., et al. Cancer Res. 67(15):7082-7087(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-406

List Size:
400 ul

List Price:

$495.00

Datasheet
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